Increasing iPSC Numbers through Systematic Culture Process Optimization in Bioreactors

Human induced pluripotent stem cells (hiPSCs) are a powerful tool for innovative approaches, such as drug discovery, in vitro disease modelling, or regenerative therapies. However, such procedures require high cell numbers to be sufficiently applicable, a criterion that is hard to satisfy with traditional 2D culture methods. Stirred-tank bioreactors on the other hand offer a 3D culture environment suitable to provide, control, and maintain optimal growth conditions for the cell of choice. In this webinar, an expert by Eppendorf will present how stirred-tank bioreactors can be utilized to systematically optimize process parameters of a hiPSC culture in a step-by-step process; an approach that led to a more than 10 × increase in cell density (almost 35 million cells/mL) compared to uncontrolled conditions while stem cell features and viability were retained.

Key learnings

• Identify the potential and challenges associated with induced pluripotent stem cells.
• Learn about the basic principles of a stirred-tank bioreactor.
• Find out how stirred-tank bioreactor-based stem cell culture can be optimized through precise parameter control.

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